Sequence

Codon optimiser

For E. coli, yeast, CHO and plants

Input sequence

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Expression host Strategy Leave the stop codon alone

—CAI before

—CAI after

—GC of the result

—Codons changed

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Custom codon usage table

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Always taking the most frequent codon makes the sequence monotonous and can worsen mRNA secondary structure; the weighted strategy produces a more natural distribution. This tool does not check secondary structure, unwanted restriction sites or repeats.