Paste a sequence. Get answers here.

Vectors

Virtual cloning

Simulate a digest and ligation, and check the construct stays in frame

Vectors

Vector feature annotator

Detect promoters, tags, protease sites and recombination sites in a sequence

Cell culture

Cell seeding and split ratio

Cells needed per vessel, suspension volume and passage arithmetic

Cell culture

Scaling between vessels

Convert a protocol written for one vessel to another by growth area

Transcriptome

Count normalisation: CPM, TPM, FPKM

Turn a raw count matrix into normalised values, with library statistics

Transcriptome

Multiple testing and volcano plot

Adjust p-values by four methods and draw a volcano plot from DESeq2 or edgeR output

Transcriptome

Transcript anatomy

Find the CDS, measure the UTRs, translate the protein and judge the Kozak context

In vivo

Tumour volume from calipers

Volume, growth curves and doubling time from caliper measurements

In vivo

Dose and injection prep

From mg/kg to the volume for each animal and the stock and vehicle needed

In vivo

Animal age and unit conversion

Age from date of birth, planning to a target age, and unit conversion

Vectors

Plasmid map and GenBank

Read and write GenBank files and draw a circular map

Sequence

Reverse complement

Complement, reverse and RNA in one click

Sequence

Translate and ORFs

Six-frame translation and open reading frames

Sequence

Restriction sites

Virtual digest and simulated gel

Sequence

Primer and Tm

Dimers, hairpins and annealing temperature

Sequence

Codon optimiser

For E. coli, yeast, CHO and plants

Protein

Protein parameters

Weight, pI, extinction and stability

Protein

Hydropathy plot

Kyte-Doolittle with adjustable window

Protein

Concentration from A280

Absorbance to mg/ml and micromolar

Calculators

Dilution

C₁V₁ and serial dilution planner

Calculators

ng to pmol

For plasmids, inserts and oligos

Calculators

RCF and RPM

With radii for common rotors

Calculators

Buffer recipes

Tris, PBS, TAE and LB at any scale

Analysis

qPCR ΔΔCt

Upload a CSV, get fold change

Analysis

Standard curve

Linear and four-parameter fitting

Analysis

Gel band size

Semi-log interpolation from your ladder