Transcriptome

Transcript anatomy

Find the CDS, measure the UTRs, translate the protein and judge the Kozak context

Input sequence

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Genetic code table CDS selection Start codon position

—Transcript length

—5′ UTR

—Coding sequence

—3′ UTR

—Protein length

—Molecular weight

—Isoelectric point

—GC content

Kozak context around the start codon

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Resulting protein

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Taking the longest ORF is a heuristic, not a fact; the real transcript may start elsewhere. If you have the CDS coordinates from a reference, use manual mode. The Kozak verdict rests only on the two positions that matter most: a purine at −3 and a G at +4.